Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)
Concentration and Motility Spermatozoa of Ayam Kampung (Gallus domesticus) in Ringer Laktat Diluents Adding Red Fruit Oil (Pandanus conoideus Lam)
DOI:
https://doi.org/10.30862/jipvet.v9i2.65Keywords:
Concentration, Motility, Spermatozoa and Red fruit oilAbstract
Red fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3), ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86% to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P <0.01) on motility 0.05). The spermatozoa concentration ranged from 1,600x106 cells / ml to 2,200x106 cells/ml. The spermatozoa concentration of treatment P1 and P2 chickens did not show any significant differences (P> 0.05), nor did among treatments P2, P3 and P4. However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil up to concentrations of more than 2% tend to reduce sperm motility.
Keywords: Concentration, Motility, Spermatozoa and Red fruit oil
Downloads
Download data is not yet available.
References
Al-Daraji, H. J. 2012a. Adding olive oil to rooster semen diluents for improving semen quality and storage ability during liquid. International Science Index, Animal and Veterinary Sciences, 6(10):862-867.
Al-Daraji, H. J. 2012b. Effect of diluent supplementation with different levels of orange juice on semen quality during liquid storage of roosters’ semen. International Journal Veteriner Science, 1(1): 5-9.
Al-Daraji, H. J. 2012c. Red wine as a diluent supplement for counteract the deleterious effects of lipid peroxidation during liquid storage of aged roosters semen. Nature and Science, 10(9):64-70.
Al-Daraji, H. J. 2013. Effect of diluent supplementation with garlic extract on semen quality of cocks during liquid storage. International Journal of Pharma and Bio Scienses, 4(3): 1260 – 1270.
Al-Daraji, H. J. 2014. Effect of diluent supplementation with tomato juice on semen quality and storage ability of roosters semen during liquid storage. International Journal of Biology, Pharmacy and Allied sciences, 1(7):918-926.
Al-Daraji, H. J. 2015. Effects of pomegranate juice as diluent supplementation on sperm quality during liquid storage of chicken semen. International Journal of Chemistry and Pharmaeutical sciences, 2(1):606-611.
Blesbois, E., Grasseau, I. and Hermier, D. 1999. Changes in lipid content of fowl spermatozoa after liquid storage at 2 to 50C. Theriogenology, 52:325–334.
Budi, M. dan Paimin, F. R. 2004. Sari Buah Merah Sebagai Obat Multi Khasiat. Jakarta: Penebar swadaya.
Bramwell, K. R., Marks, H. L. and Howarth, B. 1995. Physiology and reproduction quantitative determination of spermatozoa penetration of the perivitelline layer of the hen's ovum as assessed on oviposited eggs. Poultry Science, 74 : 1875-1883.
Chabory, E., Damon, C., Lenoir, A., Henry-Berger, J., Vernet, P., Cadet, R., et al. 2010. Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity. Journal Animal sciences, 88: 1321–1331.
Funahashi, H. and Sano, T., 2005. Selected antioxidants improve the function of extended boar semen stored at 100C. Theriogenology, 63(6): 1605-1616.
Graham, E. F. 1978. Fundamentals of the Preservation of Spermatozoa. In The Integrity of Frozen Spermatozoa. National Academy of Sciences, Washington D.C. p228.
Gunawan, I., Laksmi, D. N. D. I. dan Trilaksana, I. G. N. B. 2012. Efektivitas penambahan β-karoten dan glutathion pada bahan pengencer terhadap motilitas dan daya hidup spermatozoa pada semen beku sapi. Indonesia Medicus Veterinus, 1(3) : 385 – 393.
Hafez, E. S. E. 2000. Reproduction in Farm Animals. (7 th ed) Kiawah island, south Carolina, USA.
Indrawati, D., Bebas, W., dan Trilaksana, I. G. N. B. 2013. Motilitas dan daya hidup spermatozoa ayam kampung dengan penambahan astaxanthin pada suhu 3– 50C. Indonesia Medicus Veterinus, 2(4) : 445 – 452.
Khaeruddin., Sumantri, C., Darwati, S. dan Arifiantini, R. I. 2015. Penggunaan minyak zaitun ekstrak virgin ke dalam bahan pengencer semen terhadap kualitas spermatozoa ayam lokal. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 3(1) : 46-51.
Long, J. A. and Kramer, M. 2003. Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Sciences, (82) : 1802–1807.
Lubis, T. M. 2011. Motilitas spermatozoa ayam kampung dalam pengencer air kelapa, NaCl fisiologis dan air kelapa-NaCl fisiologis pada 25-29°C. Agripet, 11(2) : 45-50.
Murugesan, S., Matam, N., Kulkarni, R., Bhattacharya, T. K. and Chatterjee, R. N. 2013 Semen quality in white leghorn chicken selected for egg production traits turk. J. Vet. Anim. Sci, 37: 747-749.
Partyka, A., Jerysz, A., Pokorny, P. 2007. Lipid peroxidation in fresh and stored semen of green-legged partridge. Electronic Journal of Polish Agricultural Universities,10(2):08.fromhttp://www.ejpau.media.pl/volume10/issue2/art-08.html
Peters, S. O., Shoyebo, O. D., Ilori, B. M., Ozoje, M. O., Ikeobi, C. O. N. Et al. 2008. Semen quality traits of seven strain of chickens raised in the humid tropics. Int. J. Poult. Sci, 7: 949-953.
Rizal, M. dan Herdis. 2005. Daya hidup spermatozoa epididimis domba garut yang dikriopreservasi menggunakan modifikasi pengencer tris. Hayati, 12(2) : 61-66.
Solihati, N. 2008. Studi terhadap kualitas dan daya tahan hidup spermatozoa cauda epididimis domba garut menggunakan berbagai jenis pengencer. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.401-408). Bandung: Fakultas Peternakan Universitas Padjadjaran.
Sopiyana, S., Iskandar, S., Susanti, T. dan Yogaswara, D. 2006. Pengaruh krioprotektan dma, dmf dan glycerol pada proses pembekuan semen ayam kampung. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.702-708). Bogor : Balai Penelitian Ternak.
Toelihere, M. R. 1993. Inseminasi Buatan Pada Ternak. Bandung: Angkasa.
Wirahadikusumah, M. 1985. Biokimia : Metabolism Energy, Karbohidrat and Lipid. Bandung: ITB.
Yudi., Arifiantini, I., Purwantara, B. dan Yusuf, T. L. 2007. Karakteristik semen segar dan kualitas semen cair kuda dalam pengencer dimitropoulos yang disuplementasi dengan fruktosa, trehalosa dan rafinosa. Media Peternakan, 30(3) : 163-172.
Al-Daraji, H. J. 2012b. Effect of diluent supplementation with different levels of orange juice on semen quality during liquid storage of roosters’ semen. International Journal Veteriner Science, 1(1): 5-9.
Al-Daraji, H. J. 2012c. Red wine as a diluent supplement for counteract the deleterious effects of lipid peroxidation during liquid storage of aged roosters semen. Nature and Science, 10(9):64-70.
Al-Daraji, H. J. 2013. Effect of diluent supplementation with garlic extract on semen quality of cocks during liquid storage. International Journal of Pharma and Bio Scienses, 4(3): 1260 – 1270.
Al-Daraji, H. J. 2014. Effect of diluent supplementation with tomato juice on semen quality and storage ability of roosters semen during liquid storage. International Journal of Biology, Pharmacy and Allied sciences, 1(7):918-926.
Al-Daraji, H. J. 2015. Effects of pomegranate juice as diluent supplementation on sperm quality during liquid storage of chicken semen. International Journal of Chemistry and Pharmaeutical sciences, 2(1):606-611.
Blesbois, E., Grasseau, I. and Hermier, D. 1999. Changes in lipid content of fowl spermatozoa after liquid storage at 2 to 50C. Theriogenology, 52:325–334.
Budi, M. dan Paimin, F. R. 2004. Sari Buah Merah Sebagai Obat Multi Khasiat. Jakarta: Penebar swadaya.
Bramwell, K. R., Marks, H. L. and Howarth, B. 1995. Physiology and reproduction quantitative determination of spermatozoa penetration of the perivitelline layer of the hen's ovum as assessed on oviposited eggs. Poultry Science, 74 : 1875-1883.
Chabory, E., Damon, C., Lenoir, A., Henry-Berger, J., Vernet, P., Cadet, R., et al. 2010. Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity. Journal Animal sciences, 88: 1321–1331.
Funahashi, H. and Sano, T., 2005. Selected antioxidants improve the function of extended boar semen stored at 100C. Theriogenology, 63(6): 1605-1616.
Graham, E. F. 1978. Fundamentals of the Preservation of Spermatozoa. In The Integrity of Frozen Spermatozoa. National Academy of Sciences, Washington D.C. p228.
Gunawan, I., Laksmi, D. N. D. I. dan Trilaksana, I. G. N. B. 2012. Efektivitas penambahan β-karoten dan glutathion pada bahan pengencer terhadap motilitas dan daya hidup spermatozoa pada semen beku sapi. Indonesia Medicus Veterinus, 1(3) : 385 – 393.
Hafez, E. S. E. 2000. Reproduction in Farm Animals. (7 th ed) Kiawah island, south Carolina, USA.
Indrawati, D., Bebas, W., dan Trilaksana, I. G. N. B. 2013. Motilitas dan daya hidup spermatozoa ayam kampung dengan penambahan astaxanthin pada suhu 3– 50C. Indonesia Medicus Veterinus, 2(4) : 445 – 452.
Khaeruddin., Sumantri, C., Darwati, S. dan Arifiantini, R. I. 2015. Penggunaan minyak zaitun ekstrak virgin ke dalam bahan pengencer semen terhadap kualitas spermatozoa ayam lokal. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 3(1) : 46-51.
Long, J. A. and Kramer, M. 2003. Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Sciences, (82) : 1802–1807.
Lubis, T. M. 2011. Motilitas spermatozoa ayam kampung dalam pengencer air kelapa, NaCl fisiologis dan air kelapa-NaCl fisiologis pada 25-29°C. Agripet, 11(2) : 45-50.
Murugesan, S., Matam, N., Kulkarni, R., Bhattacharya, T. K. and Chatterjee, R. N. 2013 Semen quality in white leghorn chicken selected for egg production traits turk. J. Vet. Anim. Sci, 37: 747-749.
Partyka, A., Jerysz, A., Pokorny, P. 2007. Lipid peroxidation in fresh and stored semen of green-legged partridge. Electronic Journal of Polish Agricultural Universities,10(2):08.fromhttp://www.ejpau.media.pl/volume10/issue2/art-08.html
Peters, S. O., Shoyebo, O. D., Ilori, B. M., Ozoje, M. O., Ikeobi, C. O. N. Et al. 2008. Semen quality traits of seven strain of chickens raised in the humid tropics. Int. J. Poult. Sci, 7: 949-953.
Rizal, M. dan Herdis. 2005. Daya hidup spermatozoa epididimis domba garut yang dikriopreservasi menggunakan modifikasi pengencer tris. Hayati, 12(2) : 61-66.
Solihati, N. 2008. Studi terhadap kualitas dan daya tahan hidup spermatozoa cauda epididimis domba garut menggunakan berbagai jenis pengencer. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.401-408). Bandung: Fakultas Peternakan Universitas Padjadjaran.
Sopiyana, S., Iskandar, S., Susanti, T. dan Yogaswara, D. 2006. Pengaruh krioprotektan dma, dmf dan glycerol pada proses pembekuan semen ayam kampung. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.702-708). Bogor : Balai Penelitian Ternak.
Toelihere, M. R. 1993. Inseminasi Buatan Pada Ternak. Bandung: Angkasa.
Wirahadikusumah, M. 1985. Biokimia : Metabolism Energy, Karbohidrat and Lipid. Bandung: ITB.
Yudi., Arifiantini, I., Purwantara, B. dan Yusuf, T. L. 2007. Karakteristik semen segar dan kualitas semen cair kuda dalam pengencer dimitropoulos yang disuplementasi dengan fruktosa, trehalosa dan rafinosa. Media Peternakan, 30(3) : 163-172.
Downloads
Published
2019-09-22
How to Cite
Isnaeni, M., Faidiban, O. R., & Tethool, A. N. (2019). Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam): Concentration and Motility Spermatozoa of Ayam Kampung (Gallus domesticus) in Ringer Laktat Diluents Adding Red Fruit Oil (Pandanus conoideus Lam). Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 9(2), 44–49. https://doi.org/10.30862/jipvet.v9i2.65
Issue
Section
Articles
License
License and Copyright Agreement
In submitting the manuscript to the journal, the authors certify that:
- They are authorized by their co-authors to enter into these arrangements.
- The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal. Please also carefully read Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Posting Your Article Policy at https://journal.fapetunipa.ac.id/index.php/JIPVET/publicationethics
- That it is not under consideration for publication elsewhere,
- That its publication has been approved by all the author(s) and by the responsible authorities “tacitly or explicitly“ of the institutes where the work has been carried out.
- They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
- They agree to the following license and copyright agreement.
Copyright
Authors who publish with Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-NC-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
.png)
