Physicochemical quality of cow’s milk mozzarella cheese using rennet enzyme and lime juice as a natural coagulant
DOI:
https://doi.org/10.46549/jipvet.v15i3.573Keywords:
Cow's Milk, Lime Coagulant, Mozzarella Cheese, Rennet Enzym, MoistureAbstract
Cow’s milk is a natural food source rich in essential nutrients and can be processed into various value-added products, including mozzarella cheese. Although traditional mozzarella is made from buffalo milk, cow’s milk is now more commonly used due to its wider availability and lower production costs. This study evaluated the effect of lime (Citrus aurantiifolia) juice as a natural coagulant on the sensory, physical, and chemical characteristics of cow’s milk mozzarella. A Completely Randomized Design (CRD) was applied using 500 mL of cow’s milk, 15 g coarse salt, and 0.5 mL rennet enzyme per batch, with varying volumes of lime juice. Data were analyzed by ANOVA, followed by Duncan’s Multiple Range Test (DMRT) at P < 0.05. The resulting mozzarella showed soluble protein of 0.65–1.12%, moisture of 45.67–49.33%, yield of 8.80–11.00%, texture (penetrometer) of 0.05–1.58 mm/g, and meltability of 32.04–43.82%. Lime juice level significantly affected yield, texture, and meltability, but not soluble protein or moisture.
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Arief II, Hidayati N, Abidin Z, Zuraiyah TA, & Darmawati MP. 2024. Kualitas fisikokimia keju mozzarella dan soft candy dari susu sapi pada waktu pemerahan berbeda. Jurnal Sains dan Teknologi Peternakan. [Preprint]. https://doi.org/10.31605/jstp.v6i1.4333
Arifiansyah M, Wulandari E, & Chairunnisa H. 2015. Chemical characteristic (water and protein content) and acceptability of fresh cheese by using lime, lemon juice and citric acid as coagulant.
Ashshidiqi RMM, Syauqy D, Regasari R, & Putri M. 2023. Rancang bangun alat sortir keju mozzarella menggunakan metode klasifikasi naïve Bayes. Jurnal Teknologi Informasi dan Ilmu Komputer. Tersedia pada: http://j-ptiik.ub.ac.id
Auliya Z, Syarifah SM, Kafiya M, & Khumaira DA. 2023. Pembuatan keju mozzarella dengan pengasaman tidak langsung. Prosiding Seminar Nasional Penelitian dan Pengabdian Kepada Masyarakat, Universitas ’Aisyiyah Yogyakarta.
Benahmed Djilali A, Metahri MS, Lakabi L, Tahraoui H, Benseddik A, Besombes C dan Allaf K. 2024. Innovative approaches to Camembert cheese: optimizing prebiotics and coagulation conditions for enhanced quality and nutrition. Fermentation 10(10):524. https://doi.org/10.3390/fermentation10100524
Chrisna Wulandari D, & Rahmi Y. 2016. Identifikasi kesempurnaan proses pasteurisasi ditinjau dari total bakteri serta kandungan protein dan laktosa pada susu pasteurisasi kemasan produksi pabrik dan rumah tangga di Kota Batu. Majalah Kesehatan FKUB.
Dewi I, Nurzanah W, Pane Y, & Gultom T. 2024. Implementasi pengolahan susu etawa menjadi masker dalam upaya meningkatkan pendapatan kelompok ternak di Kecamatan Sunggal. Jurnal Masyarakat Mandiri (JMM) 8(2). https://doi.org/10.31764/jmm.v8i1.21815
Fox PF, & McSweeney PLH. 2007. Cheese: Chemistry, Physics and Microbiology. 3rd Edition. Springer.
Hakim L, Jambang N, Witman S, Seti Palindung L, & Puspitawati R. 2023. Meningkatkan daya mulur, daya leleh dan mikrostruktur keju mozzarella analog (sebuah review) untuk mencapai kualitas yang maksimal. Seminar Nasional Era Digital 5.0 dalam Pembangunan Teknologi Hijau Berkelanjutan. [Preprint].
Hui YH. 1991. Dairy Science and Technology Handbook. VCH Publishers, New York.
Hashim MM, Dong M, Iqbal MF, Li W, dan Chen X. 2011. Ginger protease used as coagulant enhances the proteolysis and sensory quality of Peshawari cheese compared to calf rennet. Dairy Science & Technology 91:431–440. https://doi.org/10.1007/s13594-011-0021-x
Galán E, Cabezas L, dan Fernández-Salguero J. 2012. Proteolysis, microbiology and sensory properties of ewes’ milk cheese produced with plant coagulant from cardoon Cynara cardunculus, calf rennet or a mixture thereof. International Dairy Journal 25(2):92–96. https://doi.org/10.1016/j.idairyj.2012.02.001
Maharani N, & Maharani N. 2023. Kajian penggunaan jenis rennet nabati dan hewani terhadap kualitas fisik dan kimia keju mozzarella susu sapi. Journal of Student Research (JSR). [Preprint].
Patahanny T, Hendrawati LA, & Amar H. 2019. Pembuatan keju mozzarella dengan enzim papain dan ekstrak jeruk nipis. Jurnal Teknologi Pangan.
Prastowo S, Saviera SS, Pambuko G, Vanessa R, Purwadi P, Susilowati A, & Sutarno S. 2022. Variasi genotipe dan alel gen PIT1|HinfI pada sapi perah Friesian Holstein lokal di Boyolali, Jawa Tengah. Jurnal Ilmu Peternakan dan Veteriner Tropis 12(1). https://doi.org/10.46549/jipvet.v12i1.201
Purwadi P. 2010. Rendemen keju mozzarella dengan pengasaman jeruk nipis dan enzim rennet. Jurnal Teknologi Hasil Pertanian.
Ramadhany P, Kristijarti AP, Susanto GL, & Prajna G. 2023. Physicochemical properties of tomato paste fortified functional cheddar cheese. Food ScienTech Journal 5(1): 1. https://doi.org/10.33512/fsj.v5i1.15975
Siti Aisyah, Hayuningtyas T, Wiwin W, Ramadhani EF, & Dewi ALD. 2024. Pengaruh mikroba dalam pembuatan keju dengan fermentasi asam laktat. Intellektika: Jurnal Ilmiah Mahasiswa 2(4): 48–55. https://doi.org/10.59841/intellektika.v2i4.1275
Sunarya H, Legowo AM, & Sambodho PS. 2016. Kadar air, kadar lemak dan tekstur keju mozzarella dari susu kerbau, susu sapi, dan kombinasinya. Jurnal Teknologi Pangan.
Tarapata J, Szymańska E, van der Meulen L, Miltenburg J, & Huppertz T. 2025. Moisture loss from cheese during baking: influence of cheese type, cheese mass, and temperature. Foods 14(2): 165. https://doi.org/10.3390/foods14020165
Wiedyantara AB, Rizqiati H, & Priyo Bintoro V. 2017. Aktivitas antioksidan, nilai pH, rendemen, dan tingkat kesukaan keju mozzarella dengan penambahan sari buah naga merah (Hylocereus polyrhizus). Jurnal Teknologi Pangan.
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