Genotyping Growth Hormone-AluI Locus of Bali Cattle in Pleihari, South Kalimantan
Genotyping Growth Hormone-AluI Locus of Bali Cattle in Pleihari, South Kalimantan
DOI:
https://doi.org/10.30862/jipvet.v8i1.26Keywords:
sapi Bali, genotip, growth hormone gene, Alu-I, monomorfikAbstract
Penelitian ini bertujuan untuk mengidentifikasi genotip dari lokus growth hormone-AluI (GH-AluI) pada populasi sapi Bali di Pleihari, Kalimantan Selatan. Sampel darah diambil dari 46 ekor sapi Bali (25 jantan dan 21 betina) di lokasi Pleihari, Kalimantan Selatan. Identifikasi genotip dari lokus ini dilakukan dengan mendigesti fragmen DNA berukuran 221 bp yang merentang dari daerah intron 4 (49 bp) hingga exon 5 (162 bp) menggunakan enzim restriksi AluI. Hasil penelitian menunjukkan bahwa lokus GH-AluI pada populasi sapi Bali di Pleihari, Kalimantan Selatan hanya memiliki satu macam alel, yaitu alel L, dan tidak ditemukan alel V atau alel lainnya pada lokus ini. Disuimpulkan bahwa lokus GH-AluI pada populasi sapi Bali di Pleihari, Kalimantan Selatan bersifat monomorfik.
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References
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Woychick, R.P., S.A. Camper, R.H. Lyons, S. Horowitz, E.C. Goodwin and F.M. Rottman, 1982. Cloning and Nucleotide Sequencing of the Bovine Growth Hormone Gene. Nucleic Acids Research, Vol. 10, Issue 22 7197-7210.
Zakezadeh, S., G. Rahimi, S.R. Mirae-Ashtiani, A. Nejati-Javaremi, M. Moradi-Shahrbabak, P. Reineeke, M. Reissmann, A.A. Masoudi, C. Amirinia and S.A. Mirhadi. 2006. Analysis of Bovine Growth Hormone Gene Polymorphism in Three Iranian Native Breeds and Holstein Cattle by RFLP-PCR. Biotech. 5:385-390.
Zhang, H.K., K.C. Maddock, D.R. Brown, S.K. DeNise and R.L. Ax., 1992. Bovine Growth Hormone Gene Frequencies in Samples of U.S. AI Bulls. J. Anim. Sci. 71 (Suppl.1):93 (Abstr.).
Beauchemin, Evaluation of DNA Polymorphisms involving Growth Hormone Relative to Growth and Carcass Characteristics in Brahman Steers. Genet. Mol. Res. 5(3):438-447.
Biswas, T.K., T.K. Bhattacharya, A.D. Narayan, S. Badola, P. Kumar and A. Sharma, 2003. Growth Hormone Gene Polymorphisme and Its Effect om Birth Weight in Cattle and Buffalo. Asian-Aust. J. Anim. Sci. 16(4):494-497.
Curi, R.A., H.N. Oliveira, A.C. Silveira and C.R. Lopes. 2005. Association Between IGF-I, IGF-IR and GHRH gene Polymorphism and Growth and Carcass Traits in Beef Cattle. Livestock Production Science, 94(3): 159-167.
Dybus, A., M. Kmiece, Z. Sobek and B. Winiewski. 2003. Associations between Polymorphism of the Growth Hormone Gene and Production Traits of Limousine Cattle. Available at: http://www.medwet.lublin.pl/Year%2020 03/Vol03-02/art185-02.htm. Accesion date: 10/21/05
Gordon, D.F., D.P. Quick, C.R. Erwin, J.E. Donelson and R.A. Maurer, 1983. Nucleotide Sequence of the Bovine Growth Hormone Chromosomal Gene. Molecular and Cellular Endocrinology 33(1): 81-95.
Grochowska,R., P.Sorensen, L.Zwierzchowski, M.Snochowski and P.Lovendah, 2001. Genetic Variation in Stimulated GH Release and in IGF-I of Young Dairy Cattle and Their Associations with the Leu/Val Polymorphism in the GH Gene. J. Anim. Sci 79: 450– 476.
Hartatik, T., S.. Volkandari, M.P. Rachman, and Sumadi, 2012. Polymorphism Leu/Val of Growth Hormone Gene Identified from Limmousine Cross Local Cattle in Indonesia. Sustainable Future for Human Security (SUSTAIN).
Hediger, R., S.E. Johnson, W. Barendse, R.E. Drinkwater, S.S. Moore and J. Hetzel, 1990. Assigment of the GH gene locus to 19q26qter in cattle and to 11q25qter in sheep by in situ hybridization. Genomics 8:171-174.
Jakaria, D. Duryadi, R.R. Noor, B. Tappa and H. Martojo. 2007. Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP. Media Peternakan 30: 1-10.
Jakaria and R.R. Noor, 2011. Analysis on Alu-I Growth Hormone (GHAlu-I) Gene in Bali Cattle. J. Indonesian Trop. Anim. Agric. 36(2):77-82.
Kratochvilova, M., F. Nespor and F. Urban. 2000. AluI Growth Hormone Gene Polymorphism, Lifetime Growth Parameters and Milk Production in Holstein Cattle. 51st Annual Metteng of the European Association for Animal Production, The Hague, The Netherlands.
Lucy, M.C., S.D. Hauser, P.J. Eppard, G.G. Krivi and R.J. Collier, 1991. Genetic Polymorphism within the Bovine Somatotropin (bST) Gene Detected by Polymerase Chain Reaction and Endonuclease Digestion. J. Dairy Sci. 74 (Suppl.1):284 (Abstr.).
Lucy, M.C., S.D. Hauser, P.J. Eppard, G.G. Krivi, J.H. Clark, D.E. Bauman and R.J. Collier, 1993. Variants of Somatotropin in Cattle: Gene Frequencies in Major Dairy Breeds and Associated Milk Production. Domest Anim Endocrinol. Oct; 10(4) : 325-333.
Moody, D.E., D. Pomp, S. Newman and M.D. MacNeil, 1996. Characterization of DNA Polymorphism in Three Populations of Hereford Cattle and Their Associations with Growth and Maternal EPD in Line 1 Hereford. J. Anim. Sci. 74:1784-1793.
Nei, M. and D. Kumar. 2000. Molecuar evolution and phylogenetics. Oxford University Press, New York.
Oprzadek, J., K. Flisikowski, L. Zwierchowski, E. Juszczuk-Kubiak, S. Rosochacki and E. Dymnicki. 2005. Association between Polymorphism of Some Candidate Genes and Growth Rates, Feed Intake and Utilisation, Slaughter Indicator and Meat Quality in Cattle. Archiv Fur Tierzucht, 48 (special issue): 81-87.
Pal, A., A.K. Chakravarty, T.K. Bhattacharya, B.K. Joshi and A. Sharma. 2004. Detection of Polymorphism of Growth Hormone Gene for the Analysis of Relationship between Allele Type and Growth Traits in Karan Fries cattle. Asian-Aust.J.Anim. Sci., Vol. 17, No.10 : 1334-1337.
Pereira, A.P., M.M. de Alencar, H.N. de Oliveira and L.C. de Almeida Regitano. 2005. Association of GH and IGF-1 Polymorphism with Growth Traits in a Synthetic Beef Cattle Breed. Genet. Mol. Biol. Vol.28 No.2.
Reis, C., D. Navas, M. Pereira and A. Cravador, 2001. Growth Hormone AluI Polymorphism Analysis in Eight Portuguese Bovine Breeds. Arch. Zootec. 50:41-48.
Sambrook, J., Fritcsh and T. Maniatis, 1989. Molecular Cloning Laboratory Manual. Second Edition, Vol. 3. Cold Spring Harbor Laboratory Press. USA.
Schlee, P., R. Graml, E. Schallenberger, D. Schams, O.Rottmann, A. Olbrich-Bludau and F. Pirchner, 1994a. Growth Hormone and Insulin-like Growth Factor I Concentrations in Bulls of Various Growth Hormone Genotypes. Theor Appl Genet 88 : 497-500.
Schlee, P., R. Graml, O. Rottmann and F. Pirchner, 1994b. Influence of Growth Hormone Genotypes on Breeding Values of Simmental Bulls. J. Anim Breed Genet 111:253-256.
Sutarno. 2004. Penyulihan Asam Amino Leucin oleh Valin pada Posisi 127 Gen Penyandi Hormon Pertumbuhan dan Pengaruhnya terhadap Pertumbuhan Sapi Benggala. J. Veteriner 5(1).
Unanian, M.M., C.C. Barreto, A.R. Freitas, C.M.T. Cordeiro and L.A. Josahkian. 2000. Associacao so Polimorfismo do Gene do Hormonio de Crescimentocom a Carateristica Peso em Bovinos da Raca Nelore. (Association between Growth Hormone Gene Polymorphism and Weight Traits in Nellore Bovines) In: Brasil Language; Abstract: In English. Rev. Bras. Zootec. 29 (5):1380-1386.
Vasconcellos L.P.M.K., D.T. Talhari, A.P. Pereira, L.L. Coutinho, L.C.A. Regitano, 2003. Genetic Characterization of Aberdeen Angus Cattle Using Molecular Markers. Available at: http://www.scielo.br/scielo.php?script=sci_arttex&pid=S1415-4572003 000200005&Ing=en &nrm =iso. Accession date: 12/19/03.
Woychick, R.P., S.A. Camper, R.H. Lyons, S. Horowitz, E.C. Goodwin and F.M. Rottman, 1982. Cloning and Nucleotide Sequencing of the Bovine Growth Hormone Gene. Nucleic Acids Research, Vol. 10, Issue 22 7197-7210.
Zakezadeh, S., G. Rahimi, S.R. Mirae-Ashtiani, A. Nejati-Javaremi, M. Moradi-Shahrbabak, P. Reineeke, M. Reissmann, A.A. Masoudi, C. Amirinia and S.A. Mirhadi. 2006. Analysis of Bovine Growth Hormone Gene Polymorphism in Three Iranian Native Breeds and Holstein Cattle by RFLP-PCR. Biotech. 5:385-390.
Zhang, H.K., K.C. Maddock, D.R. Brown, S.K. DeNise and R.L. Ax., 1992. Bovine Growth Hormone Gene Frequencies in Samples of U.S. AI Bulls. J. Anim. Sci. 71 (Suppl.1):93 (Abstr.).
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2018-03-01
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Mu’in, M. A. (2018). Genotyping Growth Hormone-AluI Locus of Bali Cattle in Pleihari, South Kalimantan: Genotyping Growth Hormone-AluI Locus of Bali Cattle in Pleihari, South Kalimantan. Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 8(1), 1–8. https://doi.org/10.30862/jipvet.v8i1.26
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